There’s
nothing quite like the peculiar, bone-jarring reaction of a damaged tooth
exposed to something cold: a bite of ice cream, or a cold drink, and suddenly,
that sharp, searing feeling, like a needle piercing a nerve.
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Researchers
have known for years that this phenomenon results from damage to the tooth’s
protective outer layer. But just how the message goes from the outside of your
tooth to the nerves within it has been difficult to uncover. On Friday,
biologists reported in the journal Science Advances that they have identifiedan unexpected player in this painful sensation: a protein embedded in the
surface of cells inside the teeth. The discovery provides a glimpse of the
connection between the outer world and the interior of a tooth, and could one
day help guide the development of treatments for tooth pain.
More than a
decade ago, Dr Katharina Zimmerman, now a professor at Friedrich-Alexander
University in Germany, discovered that cells producing a protein called TRPC5
were sensitive to cold. When things got chilly, TRPC5 popped open to form a
channel, allowing ions to flow across the cell’s membrane.
Ion channels
like TRPC5 are sprinkled throughout our bodies, Zimmerman said, and they are
behind some surprisingly familiar sensations. For instance, if your eyes start
to feel cold and dry in chilly air, it’s a result of an ion channel being
activated in the cornea. She wondered which other parts of the body might make
use of a cold receptor such as TRPC5. And it occurred to her that “the most
sensitive tissue in the human body can be teeth” when it comes to cold
sensations.
Within the
protective shell of their enamel, teeth are made of a hard substance called
dentin that’s threaded with tiny tunnels. At the heart of the dentin is the
tooth’s soft pulp, where nerve cells and cells called odontoblasts, which
manufacture dentin, are intertwined.
The
prevailing theory for how teeth sense cold had been that temperature changes
put pressure on the fluid in dentin’s tunnels, somehow provoking a response in
those concealed nerves. But there was little detail about how exactly that
could be happening and what could be bridging the gap between them.
Zimmerman
and her colleagues looked to see whether mice engineered to lack the TRPC5
channel still felt tooth pain as normal mice did. They were intrigued to find
that these mice, when they had damage to their teeth, did not behave as if
anything was amiss. They looked, in fact, about the same as if they had been
given an anti-inflammatory painkiller, Zimmerman said.
Her
co-author Dr Jochen Lennerz, a pathologist at Massachusetts General Hospital,
checked human teeth for signs of the ion channel and found it in their nerves
and other cells. That suggested that the channel might have a role in a
person’s perception of cold.
Over many
years, the researchers constructed a way to precisely measure the nerve signals
traveling out of a mouse’s damaged molar. They tested their ideas with
molecules that could block the activity of various channels, including TRPC5.
The picture
they slowly assembled is that TRPC5 is active in the odontoblasts. That was a
bit of a surprise, as these supporting cells are best known for making and
maintaining dentin, not aiding in perception. Within the odontoblasts, Lennerz
said, TRPC5 pops open when the signal for cold comes down the dentin tunnels,
and this results in a message being sent to the nerves.
As it
happens, one substance that keeps TRPC5 from opening is eugenol, the main
ingredient in oil of cloves, a traditional treatment for toothache. Although
the Food and Drug Administration in the United States is equivocal about
eugenol’s effectiveness, if it does lessen the pain for some people, it may be
because of its effect on TRPC5.
Perhaps the
knowledge that this channel is at the heart of cold-induced pain will lead to
better treatments for dental pain down the road — better ways to keep that message
from becoming overwhelming.
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